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0771 10x tgs bio rad  (Bio-Rad)


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    Bio-Rad 0771 10x tgs bio rad
    0771 10x Tgs Bio Rad, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 331 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/0771+10x+tgs+bio+rad/10x+Tris%2FGlycine+Buffer+for+Western+Blots+and+Native+Gels/pm41015040-261-45-48
    Average 96 stars, based on 331 article reviews
    0771 10x tgs bio rad - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Lysis:

    Article Title: Store-independent activation of STIM1-ORAI1 by SPCA2 determines the basal CFTR activity in secretory epithelial cells.
    Article Snippet: Cat# 5668; RRID: AB_10828699 SEPTIN7 polyclonal antibody ThermoFisher S. Cat# 13818-0-AP; RRID: AB_2254298 Calreticulin Invitrogen Cat# PA3-900; RRID: AB_325990 SERCA2 ATPase Monoclonal antibody Invitrogen Cat# MA3-919; RRID: AB_325502 Golgin-97 Invitrogen Cat# PA530048; RRID: AB_2547522 Lamp1 Invitrogen Cat# 53-1079-42; RRID: AB_2016657 Goat anti-Rabbit Alexa 488 ThermoFisher S. Cat# A-11034; RRID: AB_2576217 anti-GAPDH Cell Signalling Technology Cat# 5174S; RRID: AB_10622025 anti-HA-Tag Cell Signalling Technology Cat# 3724S; RRID: AB_1549585 Myc Tag Polyclonal antibody Thermo Scientific Cat# A-21281; RRID: AB_2535826 PKA alpha polyclonal antibody Invitrogene Cat# PA5-17626; RRID: AB_10985417 Donkey anti-Mouse Alexa 647 ThermoFisher S. Cat# A-31571; RRID: AB_162542 Goat anti-Chicken Alexa 647 Thermo Scientific Cat# A32933; RRID: AB_2762845 Goat anti-Rabbit Alexa 568 Thermo Scientific Cat# A-11011; RRID: AB_143157 Goat anti-Mouse Alexa 488 Thermo Scientific Cat# A-11001; RRID: AB_2534069 Chemical, peptides and recombinant proteins DMEM Gibco 41965 FBS Gibco 10500064 Kanamycin Sulfate Gibco 15160054 Antibiotic-Antimycotic Solution Gibco 15240062 Penicillin/Streptomycin Gibco 15070063 GlutaMaxTM Gibco 3505006 ATCC-formulated DMEM ATCC ATCC-30-2002 G-418 Gibco 11811031 Hygromycin B Invitrogen 10687010 Lipofectamine 2000 Invitrogen 11668019 Opti-MEM Gibco 31985070 Q5 Site-Directed Mutagenesis Kit New England BioLabs E0554S siGLO Green transfection indicator Horizon D-001630-01 Shandon Cryomatrix ThermoFisher S. 6769006 PFA Alfa Aesar 43368 PBS Sigma P4417-100TAB Sodium Citrate Sigma 71402 Tween-20 Sigma P1379 Goat Serum Sigma G9023 Bovine Serum Albumin (BSA) Pan-Biotech P061391100 (Continued on next page) ll OPEN ACCESS Current Biology 35, 1–18.e1–e7, October 20, 2025 e1 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER 1X DPBS ThermoFisher Scientific 14190250 RIPA lysis buffer (10X) EMD Millipore Corp. 20-188 cOmplete ULTRA Tablets, Mini, EASYpack Roche 05892970001 30% Acrylamide/Bis Solution 29:1 Bio-Rad 1610156 UltraPureTM TEMED Invitrogen 15524-010 NuPAGE LDS Sample Buffer (4X) Invitrogen NP0007 10X TG Bio-Rad 161-0771 10X TGS Bio-Rad 161-0772 ClarityTM Western ECL Substrate Bio-Rad 1705060 PierceTM Cell Surface Biotinylation and Isolation Kit ThermoFisher Scientific A44390 12-230 kDa Separation Module, 8 x 25 capillary cartridges Biotechne SM-W004 Anti-Rabbit Detection Module Biotechne DM-001 Anti-Mouse Detection Module Biotechne DM-002 Total Protein Detection Module Biotechne DM-TP01 RePlexTM Module Biotechne RP-001 20X Anti-Rabbit HRP Conjugate Biotechne 043-426 DynabeadsTM Protein A for Immunoprecipitation ThermoFisher Scientific 10002D Poly-L-lysine Sigma P8920-100ML Fura2-AM Invitrogen F1201 MQAE Invitrogen E3101 Mag-Fluo-4 Invitrogen M14206 BCECF-AM Invitrogen B1170 CPA Tocris 1235 CM5480 Provided by CalciMedica GSK-7975A Sigma 5.34351 Carbamylcholine chloride (Carbachol) Sigma PHR1511 Forskolin Tocris 1099 CFTRinh-172 Biotechne 3430 BAPTA-AM Sigma A1076 Methanol Sigma 34860 Two-component adhesive Picodent 1300 7100 Cell lines HeLa cell line ATCC ATCC-CCL-2 HEK-293 cell line ATCC CRL-1573 L-WRN cell line ATCC ATCC-CRL-3276 Oligonucleotides Stim1 siRNA Dharmacon L-062376-00-0005 Cftr siRNA Dharmacon L-042164-00-0005 Orai1 siRNA Dharmacon L-056431-02-0005 Spca1 siRNA Dharmacon L-041293-00-0005 Spca2 siRNA Dharmacon L-065820-01-0005 SPCA2 siRNA Dharmacon L-006280-00-0005 Septin7 siRNA Dharmacon L-042160-01-0005 Recombinant DNA ADCY1-myc-DDK ORIGENE RC237562 ADCY3-myc-DDK ORIGENE RC220272 ADCY6-myc-DDK ORIGENE RC214902 ADCY8-myc-DDK ORIGENE RC211815 (Continued on next page) ll OPEN ACCESS e2 Current Biology 35, 1–18.e1–e7, October 20, 2025 Article ..

    Western Blot:

    Article Title: Store-independent activation of STIM1-ORAI1 by SPCA2 determines the basal CFTR activity in secretory epithelial cells.
    Article Snippet: Cat# 5668; RRID: AB_10828699 SEPTIN7 polyclonal antibody ThermoFisher S. Cat# 13818-0-AP; RRID: AB_2254298 Calreticulin Invitrogen Cat# PA3-900; RRID: AB_325990 SERCA2 ATPase Monoclonal antibody Invitrogen Cat# MA3-919; RRID: AB_325502 Golgin-97 Invitrogen Cat# PA530048; RRID: AB_2547522 Lamp1 Invitrogen Cat# 53-1079-42; RRID: AB_2016657 Goat anti-Rabbit Alexa 488 ThermoFisher S. Cat# A-11034; RRID: AB_2576217 anti-GAPDH Cell Signalling Technology Cat# 5174S; RRID: AB_10622025 anti-HA-Tag Cell Signalling Technology Cat# 3724S; RRID: AB_1549585 Myc Tag Polyclonal antibody Thermo Scientific Cat# A-21281; RRID: AB_2535826 PKA alpha polyclonal antibody Invitrogene Cat# PA5-17626; RRID: AB_10985417 Donkey anti-Mouse Alexa 647 ThermoFisher S. Cat# A-31571; RRID: AB_162542 Goat anti-Chicken Alexa 647 Thermo Scientific Cat# A32933; RRID: AB_2762845 Goat anti-Rabbit Alexa 568 Thermo Scientific Cat# A-11011; RRID: AB_143157 Goat anti-Mouse Alexa 488 Thermo Scientific Cat# A-11001; RRID: AB_2534069 Chemical, peptides and recombinant proteins DMEM Gibco 41965 FBS Gibco 10500064 Kanamycin Sulfate Gibco 15160054 Antibiotic-Antimycotic Solution Gibco 15240062 Penicillin/Streptomycin Gibco 15070063 GlutaMaxTM Gibco 3505006 ATCC-formulated DMEM ATCC ATCC-30-2002 G-418 Gibco 11811031 Hygromycin B Invitrogen 10687010 Lipofectamine 2000 Invitrogen 11668019 Opti-MEM Gibco 31985070 Q5 Site-Directed Mutagenesis Kit New England BioLabs E0554S siGLO Green transfection indicator Horizon D-001630-01 Shandon Cryomatrix ThermoFisher S. 6769006 PFA Alfa Aesar 43368 PBS Sigma P4417-100TAB Sodium Citrate Sigma 71402 Tween-20 Sigma P1379 Goat Serum Sigma G9023 Bovine Serum Albumin (BSA) Pan-Biotech P061391100 (Continued on next page) ll OPEN ACCESS Current Biology 35, 1–18.e1–e7, October 20, 2025 e1 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER 1X DPBS ThermoFisher Scientific 14190250 RIPA lysis buffer (10X) EMD Millipore Corp. 20-188 cOmplete ULTRA Tablets, Mini, EASYpack Roche 05892970001 30% Acrylamide/Bis Solution 29:1 Bio-Rad 1610156 UltraPureTM TEMED Invitrogen 15524-010 NuPAGE LDS Sample Buffer (4X) Invitrogen NP0007 10X TG Bio-Rad 161-0771 10X TGS Bio-Rad 161-0772 ClarityTM Western ECL Substrate Bio-Rad 1705060 PierceTM Cell Surface Biotinylation and Isolation Kit ThermoFisher Scientific A44390 12-230 kDa Separation Module, 8 x 25 capillary cartridges Biotechne SM-W004 Anti-Rabbit Detection Module Biotechne DM-001 Anti-Mouse Detection Module Biotechne DM-002 Total Protein Detection Module Biotechne DM-TP01 RePlexTM Module Biotechne RP-001 20X Anti-Rabbit HRP Conjugate Biotechne 043-426 DynabeadsTM Protein A for Immunoprecipitation ThermoFisher Scientific 10002D Poly-L-lysine Sigma P8920-100ML Fura2-AM Invitrogen F1201 MQAE Invitrogen E3101 Mag-Fluo-4 Invitrogen M14206 BCECF-AM Invitrogen B1170 CPA Tocris 1235 CM5480 Provided by CalciMedica GSK-7975A Sigma 5.34351 Carbamylcholine chloride (Carbachol) Sigma PHR1511 Forskolin Tocris 1099 CFTRinh-172 Biotechne 3430 BAPTA-AM Sigma A1076 Methanol Sigma 34860 Two-component adhesive Picodent 1300 7100 Cell lines HeLa cell line ATCC ATCC-CCL-2 HEK-293 cell line ATCC CRL-1573 L-WRN cell line ATCC ATCC-CRL-3276 Oligonucleotides Stim1 siRNA Dharmacon L-062376-00-0005 Cftr siRNA Dharmacon L-042164-00-0005 Orai1 siRNA Dharmacon L-056431-02-0005 Spca1 siRNA Dharmacon L-041293-00-0005 Spca2 siRNA Dharmacon L-065820-01-0005 SPCA2 siRNA Dharmacon L-006280-00-0005 Septin7 siRNA Dharmacon L-042160-01-0005 Recombinant DNA ADCY1-myc-DDK ORIGENE RC237562 ADCY3-myc-DDK ORIGENE RC220272 ADCY6-myc-DDK ORIGENE RC214902 ADCY8-myc-DDK ORIGENE RC211815 (Continued on next page) ll OPEN ACCESS e2 Current Biology 35, 1–18.e1–e7, October 20, 2025 Article ..

    Article Title: Orai1 calcium channel inhibition prevents progression of chronic pancreatitis
    Article Snippet: .. Supplemental Table 1 – Materials used during experiments listed (A-D) Name Provider Cat. No. 10x TG Buffer Bio-Rad 161-0771 10X TGS Bio-Rad 161-0772 16% Formaldehyde Thermo Scientific 28906 16% Paraformaldehyde Alfa Aesar 11400580 2-Mercaptoethanol Sigma-Aldrich M6250-100ML 30% Acrylamide/Bis Solution 29:1 Bio-Rad 1610156 5-Bromo-2 Deoxyuridine Invitrogen B23151 96% Ethanol Molar Chemicals 02911-481-410 Acetic acid Sigma-Aldrich A6283-1L Alpha-Amylase Assay kit Diagnosticum 47462 Alum hematoxylin Reanal 08004-6-25 Ammonium Persulphate Thermo Scientific 17874 Anilin blue Thomasker 13381-25G Apoptosis/Necrosis Assay kit (blue/green/red) Abcam ab176749 BCECF,AM Invitrogen B1170 BondTM Dewax Solution Leica Biosystems AR9222 BondTM Epitope Retrieval Leica Biosystems AR9640 BondTM Polymer Refine Detection Leica Biosystems DS9800 BondTM Primary Antibody Diluent Leica Biosystems AR9352 BondTM Wash Solution Leica Biosystems AR9590 Boric acid Sigma-Aldrich B6768-1KG Bovine Serum Albumin Pan-Biotech P06-1391500 Bromophenol Blue Sigma-Aldrich B0126-25G Caerulein ammonium salt Bachem AG 4030451.0005 Clarity Western ECL Substrate Bio-Rad 170-5060 CM5480 Provided by CalciMedica Collagenase Worthington LS005273 Collagenase IV Gibco 17104019 cOmplete ULTRA protease inh. cocktail Roche 05892970001 Coomassie® Brilliant Blue Merck 1.15444.0025 Corn cob bedding - REHOFIX JRS REHOFIX MK2000 CPA Tocris 1235 Crystal Violet Sigma-Aldrich C0775 DAPI Sigma-Alrich MBD0015-5ML DMEM/F12 Sigma-Aldrich D6421 DPBS (1X) Gibco 14190-094 Supplemental Table 2 – Materials used during experiments listed (E-R) Name Provider Cat.No. .. EnzCheckTM Elastase Assay kit Invitrogen E12056 Eosin Y Alcoholic Solution Leica Biosystems 3801600E Eukitt® Quick-hardening mounting medium Sigma-Aldrich 03989-100ML FluoromountTM Aqueous Mounting Medium Sigma-Aldrich F4680 Fuchsin acid Gyógyszeralapanyag Készletező V. 2-06-073-4-15 FURA-2,AM Invitrogen F1201 Glycerol Sigma-Aldrich G5516-1L Hank’s Balanced Salt Solution (HBSS) Sigma-Aldrich H9269 Harrys Hematoxylin Leica Biosystems 3801560E HEPES Sigma-Aldrich H3375 Hydrochloric Acid (HCl) Sigma-Aldrich 258148 Hydroxyproline Assay kit Sigma-Aldrich MAK008 iScriptTM cDNA Synthesis Kit Bio-Rad 178890 Isopropyl alcohol Sigma-Aldrich 19516-500ML Ketamine - - Media 199 Gibco 11150059 Methanol Sigma-Aldrich 34860 MQAE Invitrogen E3101 Novocastra Peroxidase Block Leica Biosystems RE7101-CE NucleoSpin RNA Plus kit Macherey-Nagel 740955.250 NucleoSpin totalRNA FFPE XS Kit Macherey-Nagel 740969 NucleoZOL Macherey-Nagel 740404.200 Oil Red O Stain Kit Abcam ab150678 Orange G Reanal 16230 Pentobarbital - - Phosphoric Acid Sigma-Aldrich 30416-1L-M Phosphotungstic acid Reanal 06101 PhosSTOP phosphatase inhibitor Roche 04906845001 Picric acid Sigma-Aldrich 197378 Pierce BCA Protein Assay Kit Thermo Scientific 23225 PluronicTM F-127 Invitrogen P3000MP Poly-L-lysine Sigma-Aldrich P4707-50ML Precision Plus Protein WesternC Standards Bio-Rad 161-0376 RIPA Lysis Buffer Merck Millipore 20-188 RNAlaterTM Sigma-Aldrich R0901-100ML Supplemental Table 3 – Materials used during experiments listed (S-X) Name Provider Cat.No.

    Isolation:

    Article Title: Store-independent activation of STIM1-ORAI1 by SPCA2 determines the basal CFTR activity in secretory epithelial cells.
    Article Snippet: Cat# 5668; RRID: AB_10828699 SEPTIN7 polyclonal antibody ThermoFisher S. Cat# 13818-0-AP; RRID: AB_2254298 Calreticulin Invitrogen Cat# PA3-900; RRID: AB_325990 SERCA2 ATPase Monoclonal antibody Invitrogen Cat# MA3-919; RRID: AB_325502 Golgin-97 Invitrogen Cat# PA530048; RRID: AB_2547522 Lamp1 Invitrogen Cat# 53-1079-42; RRID: AB_2016657 Goat anti-Rabbit Alexa 488 ThermoFisher S. Cat# A-11034; RRID: AB_2576217 anti-GAPDH Cell Signalling Technology Cat# 5174S; RRID: AB_10622025 anti-HA-Tag Cell Signalling Technology Cat# 3724S; RRID: AB_1549585 Myc Tag Polyclonal antibody Thermo Scientific Cat# A-21281; RRID: AB_2535826 PKA alpha polyclonal antibody Invitrogene Cat# PA5-17626; RRID: AB_10985417 Donkey anti-Mouse Alexa 647 ThermoFisher S. Cat# A-31571; RRID: AB_162542 Goat anti-Chicken Alexa 647 Thermo Scientific Cat# A32933; RRID: AB_2762845 Goat anti-Rabbit Alexa 568 Thermo Scientific Cat# A-11011; RRID: AB_143157 Goat anti-Mouse Alexa 488 Thermo Scientific Cat# A-11001; RRID: AB_2534069 Chemical, peptides and recombinant proteins DMEM Gibco 41965 FBS Gibco 10500064 Kanamycin Sulfate Gibco 15160054 Antibiotic-Antimycotic Solution Gibco 15240062 Penicillin/Streptomycin Gibco 15070063 GlutaMaxTM Gibco 3505006 ATCC-formulated DMEM ATCC ATCC-30-2002 G-418 Gibco 11811031 Hygromycin B Invitrogen 10687010 Lipofectamine 2000 Invitrogen 11668019 Opti-MEM Gibco 31985070 Q5 Site-Directed Mutagenesis Kit New England BioLabs E0554S siGLO Green transfection indicator Horizon D-001630-01 Shandon Cryomatrix ThermoFisher S. 6769006 PFA Alfa Aesar 43368 PBS Sigma P4417-100TAB Sodium Citrate Sigma 71402 Tween-20 Sigma P1379 Goat Serum Sigma G9023 Bovine Serum Albumin (BSA) Pan-Biotech P061391100 (Continued on next page) ll OPEN ACCESS Current Biology 35, 1–18.e1–e7, October 20, 2025 e1 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER 1X DPBS ThermoFisher Scientific 14190250 RIPA lysis buffer (10X) EMD Millipore Corp. 20-188 cOmplete ULTRA Tablets, Mini, EASYpack Roche 05892970001 30% Acrylamide/Bis Solution 29:1 Bio-Rad 1610156 UltraPureTM TEMED Invitrogen 15524-010 NuPAGE LDS Sample Buffer (4X) Invitrogen NP0007 10X TG Bio-Rad 161-0771 10X TGS Bio-Rad 161-0772 ClarityTM Western ECL Substrate Bio-Rad 1705060 PierceTM Cell Surface Biotinylation and Isolation Kit ThermoFisher Scientific A44390 12-230 kDa Separation Module, 8 x 25 capillary cartridges Biotechne SM-W004 Anti-Rabbit Detection Module Biotechne DM-001 Anti-Mouse Detection Module Biotechne DM-002 Total Protein Detection Module Biotechne DM-TP01 RePlexTM Module Biotechne RP-001 20X Anti-Rabbit HRP Conjugate Biotechne 043-426 DynabeadsTM Protein A for Immunoprecipitation ThermoFisher Scientific 10002D Poly-L-lysine Sigma P8920-100ML Fura2-AM Invitrogen F1201 MQAE Invitrogen E3101 Mag-Fluo-4 Invitrogen M14206 BCECF-AM Invitrogen B1170 CPA Tocris 1235 CM5480 Provided by CalciMedica GSK-7975A Sigma 5.34351 Carbamylcholine chloride (Carbachol) Sigma PHR1511 Forskolin Tocris 1099 CFTRinh-172 Biotechne 3430 BAPTA-AM Sigma A1076 Methanol Sigma 34860 Two-component adhesive Picodent 1300 7100 Cell lines HeLa cell line ATCC ATCC-CCL-2 HEK-293 cell line ATCC CRL-1573 L-WRN cell line ATCC ATCC-CRL-3276 Oligonucleotides Stim1 siRNA Dharmacon L-062376-00-0005 Cftr siRNA Dharmacon L-042164-00-0005 Orai1 siRNA Dharmacon L-056431-02-0005 Spca1 siRNA Dharmacon L-041293-00-0005 Spca2 siRNA Dharmacon L-065820-01-0005 SPCA2 siRNA Dharmacon L-006280-00-0005 Septin7 siRNA Dharmacon L-042160-01-0005 Recombinant DNA ADCY1-myc-DDK ORIGENE RC237562 ADCY3-myc-DDK ORIGENE RC220272 ADCY6-myc-DDK ORIGENE RC214902 ADCY8-myc-DDK ORIGENE RC211815 (Continued on next page) ll OPEN ACCESS e2 Current Biology 35, 1–18.e1–e7, October 20, 2025 Article ..

    Immunoprecipitation:

    Article Title: Store-independent activation of STIM1-ORAI1 by SPCA2 determines the basal CFTR activity in secretory epithelial cells.
    Article Snippet: Cat# 5668; RRID: AB_10828699 SEPTIN7 polyclonal antibody ThermoFisher S. Cat# 13818-0-AP; RRID: AB_2254298 Calreticulin Invitrogen Cat# PA3-900; RRID: AB_325990 SERCA2 ATPase Monoclonal antibody Invitrogen Cat# MA3-919; RRID: AB_325502 Golgin-97 Invitrogen Cat# PA530048; RRID: AB_2547522 Lamp1 Invitrogen Cat# 53-1079-42; RRID: AB_2016657 Goat anti-Rabbit Alexa 488 ThermoFisher S. Cat# A-11034; RRID: AB_2576217 anti-GAPDH Cell Signalling Technology Cat# 5174S; RRID: AB_10622025 anti-HA-Tag Cell Signalling Technology Cat# 3724S; RRID: AB_1549585 Myc Tag Polyclonal antibody Thermo Scientific Cat# A-21281; RRID: AB_2535826 PKA alpha polyclonal antibody Invitrogene Cat# PA5-17626; RRID: AB_10985417 Donkey anti-Mouse Alexa 647 ThermoFisher S. Cat# A-31571; RRID: AB_162542 Goat anti-Chicken Alexa 647 Thermo Scientific Cat# A32933; RRID: AB_2762845 Goat anti-Rabbit Alexa 568 Thermo Scientific Cat# A-11011; RRID: AB_143157 Goat anti-Mouse Alexa 488 Thermo Scientific Cat# A-11001; RRID: AB_2534069 Chemical, peptides and recombinant proteins DMEM Gibco 41965 FBS Gibco 10500064 Kanamycin Sulfate Gibco 15160054 Antibiotic-Antimycotic Solution Gibco 15240062 Penicillin/Streptomycin Gibco 15070063 GlutaMaxTM Gibco 3505006 ATCC-formulated DMEM ATCC ATCC-30-2002 G-418 Gibco 11811031 Hygromycin B Invitrogen 10687010 Lipofectamine 2000 Invitrogen 11668019 Opti-MEM Gibco 31985070 Q5 Site-Directed Mutagenesis Kit New England BioLabs E0554S siGLO Green transfection indicator Horizon D-001630-01 Shandon Cryomatrix ThermoFisher S. 6769006 PFA Alfa Aesar 43368 PBS Sigma P4417-100TAB Sodium Citrate Sigma 71402 Tween-20 Sigma P1379 Goat Serum Sigma G9023 Bovine Serum Albumin (BSA) Pan-Biotech P061391100 (Continued on next page) ll OPEN ACCESS Current Biology 35, 1–18.e1–e7, October 20, 2025 e1 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER 1X DPBS ThermoFisher Scientific 14190250 RIPA lysis buffer (10X) EMD Millipore Corp. 20-188 cOmplete ULTRA Tablets, Mini, EASYpack Roche 05892970001 30% Acrylamide/Bis Solution 29:1 Bio-Rad 1610156 UltraPureTM TEMED Invitrogen 15524-010 NuPAGE LDS Sample Buffer (4X) Invitrogen NP0007 10X TG Bio-Rad 161-0771 10X TGS Bio-Rad 161-0772 ClarityTM Western ECL Substrate Bio-Rad 1705060 PierceTM Cell Surface Biotinylation and Isolation Kit ThermoFisher Scientific A44390 12-230 kDa Separation Module, 8 x 25 capillary cartridges Biotechne SM-W004 Anti-Rabbit Detection Module Biotechne DM-001 Anti-Mouse Detection Module Biotechne DM-002 Total Protein Detection Module Biotechne DM-TP01 RePlexTM Module Biotechne RP-001 20X Anti-Rabbit HRP Conjugate Biotechne 043-426 DynabeadsTM Protein A for Immunoprecipitation ThermoFisher Scientific 10002D Poly-L-lysine Sigma P8920-100ML Fura2-AM Invitrogen F1201 MQAE Invitrogen E3101 Mag-Fluo-4 Invitrogen M14206 BCECF-AM Invitrogen B1170 CPA Tocris 1235 CM5480 Provided by CalciMedica GSK-7975A Sigma 5.34351 Carbamylcholine chloride (Carbachol) Sigma PHR1511 Forskolin Tocris 1099 CFTRinh-172 Biotechne 3430 BAPTA-AM Sigma A1076 Methanol Sigma 34860 Two-component adhesive Picodent 1300 7100 Cell lines HeLa cell line ATCC ATCC-CCL-2 HEK-293 cell line ATCC CRL-1573 L-WRN cell line ATCC ATCC-CRL-3276 Oligonucleotides Stim1 siRNA Dharmacon L-062376-00-0005 Cftr siRNA Dharmacon L-042164-00-0005 Orai1 siRNA Dharmacon L-056431-02-0005 Spca1 siRNA Dharmacon L-041293-00-0005 Spca2 siRNA Dharmacon L-065820-01-0005 SPCA2 siRNA Dharmacon L-006280-00-0005 Septin7 siRNA Dharmacon L-042160-01-0005 Recombinant DNA ADCY1-myc-DDK ORIGENE RC237562 ADCY3-myc-DDK ORIGENE RC220272 ADCY6-myc-DDK ORIGENE RC214902 ADCY8-myc-DDK ORIGENE RC211815 (Continued on next page) ll OPEN ACCESS e2 Current Biology 35, 1–18.e1–e7, October 20, 2025 Article ..

    Adhesive:

    Article Title: Store-independent activation of STIM1-ORAI1 by SPCA2 determines the basal CFTR activity in secretory epithelial cells.
    Article Snippet: Cat# 5668; RRID: AB_10828699 SEPTIN7 polyclonal antibody ThermoFisher S. Cat# 13818-0-AP; RRID: AB_2254298 Calreticulin Invitrogen Cat# PA3-900; RRID: AB_325990 SERCA2 ATPase Monoclonal antibody Invitrogen Cat# MA3-919; RRID: AB_325502 Golgin-97 Invitrogen Cat# PA530048; RRID: AB_2547522 Lamp1 Invitrogen Cat# 53-1079-42; RRID: AB_2016657 Goat anti-Rabbit Alexa 488 ThermoFisher S. Cat# A-11034; RRID: AB_2576217 anti-GAPDH Cell Signalling Technology Cat# 5174S; RRID: AB_10622025 anti-HA-Tag Cell Signalling Technology Cat# 3724S; RRID: AB_1549585 Myc Tag Polyclonal antibody Thermo Scientific Cat# A-21281; RRID: AB_2535826 PKA alpha polyclonal antibody Invitrogene Cat# PA5-17626; RRID: AB_10985417 Donkey anti-Mouse Alexa 647 ThermoFisher S. Cat# A-31571; RRID: AB_162542 Goat anti-Chicken Alexa 647 Thermo Scientific Cat# A32933; RRID: AB_2762845 Goat anti-Rabbit Alexa 568 Thermo Scientific Cat# A-11011; RRID: AB_143157 Goat anti-Mouse Alexa 488 Thermo Scientific Cat# A-11001; RRID: AB_2534069 Chemical, peptides and recombinant proteins DMEM Gibco 41965 FBS Gibco 10500064 Kanamycin Sulfate Gibco 15160054 Antibiotic-Antimycotic Solution Gibco 15240062 Penicillin/Streptomycin Gibco 15070063 GlutaMaxTM Gibco 3505006 ATCC-formulated DMEM ATCC ATCC-30-2002 G-418 Gibco 11811031 Hygromycin B Invitrogen 10687010 Lipofectamine 2000 Invitrogen 11668019 Opti-MEM Gibco 31985070 Q5 Site-Directed Mutagenesis Kit New England BioLabs E0554S siGLO Green transfection indicator Horizon D-001630-01 Shandon Cryomatrix ThermoFisher S. 6769006 PFA Alfa Aesar 43368 PBS Sigma P4417-100TAB Sodium Citrate Sigma 71402 Tween-20 Sigma P1379 Goat Serum Sigma G9023 Bovine Serum Albumin (BSA) Pan-Biotech P061391100 (Continued on next page) ll OPEN ACCESS Current Biology 35, 1–18.e1–e7, October 20, 2025 e1 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER 1X DPBS ThermoFisher Scientific 14190250 RIPA lysis buffer (10X) EMD Millipore Corp. 20-188 cOmplete ULTRA Tablets, Mini, EASYpack Roche 05892970001 30% Acrylamide/Bis Solution 29:1 Bio-Rad 1610156 UltraPureTM TEMED Invitrogen 15524-010 NuPAGE LDS Sample Buffer (4X) Invitrogen NP0007 10X TG Bio-Rad 161-0771 10X TGS Bio-Rad 161-0772 ClarityTM Western ECL Substrate Bio-Rad 1705060 PierceTM Cell Surface Biotinylation and Isolation Kit ThermoFisher Scientific A44390 12-230 kDa Separation Module, 8 x 25 capillary cartridges Biotechne SM-W004 Anti-Rabbit Detection Module Biotechne DM-001 Anti-Mouse Detection Module Biotechne DM-002 Total Protein Detection Module Biotechne DM-TP01 RePlexTM Module Biotechne RP-001 20X Anti-Rabbit HRP Conjugate Biotechne 043-426 DynabeadsTM Protein A for Immunoprecipitation ThermoFisher Scientific 10002D Poly-L-lysine Sigma P8920-100ML Fura2-AM Invitrogen F1201 MQAE Invitrogen E3101 Mag-Fluo-4 Invitrogen M14206 BCECF-AM Invitrogen B1170 CPA Tocris 1235 CM5480 Provided by CalciMedica GSK-7975A Sigma 5.34351 Carbamylcholine chloride (Carbachol) Sigma PHR1511 Forskolin Tocris 1099 CFTRinh-172 Biotechne 3430 BAPTA-AM Sigma A1076 Methanol Sigma 34860 Two-component adhesive Picodent 1300 7100 Cell lines HeLa cell line ATCC ATCC-CCL-2 HEK-293 cell line ATCC CRL-1573 L-WRN cell line ATCC ATCC-CRL-3276 Oligonucleotides Stim1 siRNA Dharmacon L-062376-00-0005 Cftr siRNA Dharmacon L-042164-00-0005 Orai1 siRNA Dharmacon L-056431-02-0005 Spca1 siRNA Dharmacon L-041293-00-0005 Spca2 siRNA Dharmacon L-065820-01-0005 SPCA2 siRNA Dharmacon L-006280-00-0005 Septin7 siRNA Dharmacon L-042160-01-0005 Recombinant DNA ADCY1-myc-DDK ORIGENE RC237562 ADCY3-myc-DDK ORIGENE RC220272 ADCY6-myc-DDK ORIGENE RC214902 ADCY8-myc-DDK ORIGENE RC211815 (Continued on next page) ll OPEN ACCESS e2 Current Biology 35, 1–18.e1–e7, October 20, 2025 Article ..

    Recombinant:

    Article Title: Store-independent activation of STIM1-ORAI1 by SPCA2 determines the basal CFTR activity in secretory epithelial cells.
    Article Snippet: Cat# 5668; RRID: AB_10828699 SEPTIN7 polyclonal antibody ThermoFisher S. Cat# 13818-0-AP; RRID: AB_2254298 Calreticulin Invitrogen Cat# PA3-900; RRID: AB_325990 SERCA2 ATPase Monoclonal antibody Invitrogen Cat# MA3-919; RRID: AB_325502 Golgin-97 Invitrogen Cat# PA530048; RRID: AB_2547522 Lamp1 Invitrogen Cat# 53-1079-42; RRID: AB_2016657 Goat anti-Rabbit Alexa 488 ThermoFisher S. Cat# A-11034; RRID: AB_2576217 anti-GAPDH Cell Signalling Technology Cat# 5174S; RRID: AB_10622025 anti-HA-Tag Cell Signalling Technology Cat# 3724S; RRID: AB_1549585 Myc Tag Polyclonal antibody Thermo Scientific Cat# A-21281; RRID: AB_2535826 PKA alpha polyclonal antibody Invitrogene Cat# PA5-17626; RRID: AB_10985417 Donkey anti-Mouse Alexa 647 ThermoFisher S. Cat# A-31571; RRID: AB_162542 Goat anti-Chicken Alexa 647 Thermo Scientific Cat# A32933; RRID: AB_2762845 Goat anti-Rabbit Alexa 568 Thermo Scientific Cat# A-11011; RRID: AB_143157 Goat anti-Mouse Alexa 488 Thermo Scientific Cat# A-11001; RRID: AB_2534069 Chemical, peptides and recombinant proteins DMEM Gibco 41965 FBS Gibco 10500064 Kanamycin Sulfate Gibco 15160054 Antibiotic-Antimycotic Solution Gibco 15240062 Penicillin/Streptomycin Gibco 15070063 GlutaMaxTM Gibco 3505006 ATCC-formulated DMEM ATCC ATCC-30-2002 G-418 Gibco 11811031 Hygromycin B Invitrogen 10687010 Lipofectamine 2000 Invitrogen 11668019 Opti-MEM Gibco 31985070 Q5 Site-Directed Mutagenesis Kit New England BioLabs E0554S siGLO Green transfection indicator Horizon D-001630-01 Shandon Cryomatrix ThermoFisher S. 6769006 PFA Alfa Aesar 43368 PBS Sigma P4417-100TAB Sodium Citrate Sigma 71402 Tween-20 Sigma P1379 Goat Serum Sigma G9023 Bovine Serum Albumin (BSA) Pan-Biotech P061391100 (Continued on next page) ll OPEN ACCESS Current Biology 35, 1–18.e1–e7, October 20, 2025 e1 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER 1X DPBS ThermoFisher Scientific 14190250 RIPA lysis buffer (10X) EMD Millipore Corp. 20-188 cOmplete ULTRA Tablets, Mini, EASYpack Roche 05892970001 30% Acrylamide/Bis Solution 29:1 Bio-Rad 1610156 UltraPureTM TEMED Invitrogen 15524-010 NuPAGE LDS Sample Buffer (4X) Invitrogen NP0007 10X TG Bio-Rad 161-0771 10X TGS Bio-Rad 161-0772 ClarityTM Western ECL Substrate Bio-Rad 1705060 PierceTM Cell Surface Biotinylation and Isolation Kit ThermoFisher Scientific A44390 12-230 kDa Separation Module, 8 x 25 capillary cartridges Biotechne SM-W004 Anti-Rabbit Detection Module Biotechne DM-001 Anti-Mouse Detection Module Biotechne DM-002 Total Protein Detection Module Biotechne DM-TP01 RePlexTM Module Biotechne RP-001 20X Anti-Rabbit HRP Conjugate Biotechne 043-426 DynabeadsTM Protein A for Immunoprecipitation ThermoFisher Scientific 10002D Poly-L-lysine Sigma P8920-100ML Fura2-AM Invitrogen F1201 MQAE Invitrogen E3101 Mag-Fluo-4 Invitrogen M14206 BCECF-AM Invitrogen B1170 CPA Tocris 1235 CM5480 Provided by CalciMedica GSK-7975A Sigma 5.34351 Carbamylcholine chloride (Carbachol) Sigma PHR1511 Forskolin Tocris 1099 CFTRinh-172 Biotechne 3430 BAPTA-AM Sigma A1076 Methanol Sigma 34860 Two-component adhesive Picodent 1300 7100 Cell lines HeLa cell line ATCC ATCC-CCL-2 HEK-293 cell line ATCC CRL-1573 L-WRN cell line ATCC ATCC-CRL-3276 Oligonucleotides Stim1 siRNA Dharmacon L-062376-00-0005 Cftr siRNA Dharmacon L-042164-00-0005 Orai1 siRNA Dharmacon L-056431-02-0005 Spca1 siRNA Dharmacon L-041293-00-0005 Spca2 siRNA Dharmacon L-065820-01-0005 SPCA2 siRNA Dharmacon L-006280-00-0005 Septin7 siRNA Dharmacon L-042160-01-0005 Recombinant DNA ADCY1-myc-DDK ORIGENE RC237562 ADCY3-myc-DDK ORIGENE RC220272 ADCY6-myc-DDK ORIGENE RC214902 ADCY8-myc-DDK ORIGENE RC211815 (Continued on next page) ll OPEN ACCESS e2 Current Biology 35, 1–18.e1–e7, October 20, 2025 Article ..

    Polymer:

    Article Title: Orai1 calcium channel inhibition prevents progression of chronic pancreatitis
    Article Snippet: .. Supplemental Table 1 – Materials used during experiments listed (A-D) Name Provider Cat. No. 10x TG Buffer Bio-Rad 161-0771 10X TGS Bio-Rad 161-0772 16% Formaldehyde Thermo Scientific 28906 16% Paraformaldehyde Alfa Aesar 11400580 2-Mercaptoethanol Sigma-Aldrich M6250-100ML 30% Acrylamide/Bis Solution 29:1 Bio-Rad 1610156 5-Bromo-2 Deoxyuridine Invitrogen B23151 96% Ethanol Molar Chemicals 02911-481-410 Acetic acid Sigma-Aldrich A6283-1L Alpha-Amylase Assay kit Diagnosticum 47462 Alum hematoxylin Reanal 08004-6-25 Ammonium Persulphate Thermo Scientific 17874 Anilin blue Thomasker 13381-25G Apoptosis/Necrosis Assay kit (blue/green/red) Abcam ab176749 BCECF,AM Invitrogen B1170 BondTM Dewax Solution Leica Biosystems AR9222 BondTM Epitope Retrieval Leica Biosystems AR9640 BondTM Polymer Refine Detection Leica Biosystems DS9800 BondTM Primary Antibody Diluent Leica Biosystems AR9352 BondTM Wash Solution Leica Biosystems AR9590 Boric acid Sigma-Aldrich B6768-1KG Bovine Serum Albumin Pan-Biotech P06-1391500 Bromophenol Blue Sigma-Aldrich B0126-25G Caerulein ammonium salt Bachem AG 4030451.0005 Clarity Western ECL Substrate Bio-Rad 170-5060 CM5480 Provided by CalciMedica Collagenase Worthington LS005273 Collagenase IV Gibco 17104019 cOmplete ULTRA protease inh. cocktail Roche 05892970001 Coomassie® Brilliant Blue Merck 1.15444.0025 Corn cob bedding - REHOFIX JRS REHOFIX MK2000 CPA Tocris 1235 Crystal Violet Sigma-Aldrich C0775 DAPI Sigma-Alrich MBD0015-5ML DMEM/F12 Sigma-Aldrich D6421 DPBS (1X) Gibco 14190-094 Supplemental Table 2 – Materials used during experiments listed (E-R) Name Provider Cat.No. .. EnzCheckTM Elastase Assay kit Invitrogen E12056 Eosin Y Alcoholic Solution Leica Biosystems 3801600E Eukitt® Quick-hardening mounting medium Sigma-Aldrich 03989-100ML FluoromountTM Aqueous Mounting Medium Sigma-Aldrich F4680 Fuchsin acid Gyógyszeralapanyag Készletező V. 2-06-073-4-15 FURA-2,AM Invitrogen F1201 Glycerol Sigma-Aldrich G5516-1L Hank’s Balanced Salt Solution (HBSS) Sigma-Aldrich H9269 Harrys Hematoxylin Leica Biosystems 3801560E HEPES Sigma-Aldrich H3375 Hydrochloric Acid (HCl) Sigma-Aldrich 258148 Hydroxyproline Assay kit Sigma-Aldrich MAK008 iScriptTM cDNA Synthesis Kit Bio-Rad 178890 Isopropyl alcohol Sigma-Aldrich 19516-500ML Ketamine - - Media 199 Gibco 11150059 Methanol Sigma-Aldrich 34860 MQAE Invitrogen E3101 Novocastra Peroxidase Block Leica Biosystems RE7101-CE NucleoSpin RNA Plus kit Macherey-Nagel 740955.250 NucleoSpin totalRNA FFPE XS Kit Macherey-Nagel 740969 NucleoZOL Macherey-Nagel 740404.200 Oil Red O Stain Kit Abcam ab150678 Orange G Reanal 16230 Pentobarbital - - Phosphoric Acid Sigma-Aldrich 30416-1L-M Phosphotungstic acid Reanal 06101 PhosSTOP phosphatase inhibitor Roche 04906845001 Picric acid Sigma-Aldrich 197378 Pierce BCA Protein Assay Kit Thermo Scientific 23225 PluronicTM F-127 Invitrogen P3000MP Poly-L-lysine Sigma-Aldrich P4707-50ML Precision Plus Protein WesternC Standards Bio-Rad 161-0376 RIPA Lysis Buffer Merck Millipore 20-188 RNAlaterTM Sigma-Aldrich R0901-100ML Supplemental Table 3 – Materials used during experiments listed (S-X) Name Provider Cat.No.



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